Food or beverage additive containing fucoidan and food and beverage containing fucoidan

ABSTRACT

Food or beverage where fucoidan which is derived from a fucoidan-containing substance is contained therein, added thereto and/or diluted therein. Food or beverage where fucoidan which is derived from a fucoidan-containing substance and where algins are reduced or eliminated, is contained therein. Apoptosis-inducing food or beverage where an effective amount of fucoidan having an apoptosis-inducing ability is contained therein.

This application is a Continuation Application of Ser. No. 09/180,465,filed Nov. 9, 1998, now abandoned, which is a 371 of PCT/JP97/01664,filed May 15, 1997, pending.

FIELD OF THE INVENTION

The present invention relates to food or beverage which containsfucoidan and has an excellent physiological effect as well.

DESCRIPTION OF THE RELATED ART

Fucoidan is a polysaccharide containing sulfated fucose which iscontained in seaweed, trepang, etc. No attempt for researching anddeveloping fucoidan and for positively using it in food and beverage hasbeen known yet.

The object of the present invention is to offer fucoidan-containingmaterials, and to offer fucoidan-containing food and beverage having aphysiological activity which is useful for good health and having anexcellent taste.

SUMMARY OF THE INVENTION

The present invention will be summarized as follows. The first featureof the present invention relates to food or beverage which containsfucoidan derived from fucoidan-containing substances.

The second feature of the present invention relates to food or beveragewhich contains fucoidan wherein algins derived from thefricoidan-containing substances are reduced or removed.

The third feature of the present invention relates to apoptosis-inducingfood or beverage which contains an effective amount of fucoidan havingan apoptosis-inducing ability derived from the fucoidan-containingsubstances.

The fourth feature of the present invention relates toapoptosis-inducing food or beverage which contains an effective amountof fucoidan having an apoptosis-inducing ability wherein algins derivedfrom the fucoidan-containing substances are reduced or removed.

Incidentally, the term “algins” used in this specification stands foralginic acid and its salts and esters as well as degraded productsthereof.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the precipitate forming rates of fucoidan-U and fucoidan-F.

FIG. 2 shows an apoptosis-inducing action of an extract (0.2 mg/ml)derived from seaweed.

FIG. 3 shows an apoptosis-inducing action of an extract (0.5 mg/ml)derived from seaweed.

FIG. 4 shows an apoptosis-inducing action of an extract (1 mg/ml)derived from seaweed.

FIG. 5 shows an apoptosis-inducing action of fucoidan-U and fucoidan-F.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

The present invention will be more specifically described below.

Fucoidan can be prepared from seaweed, trepang, etc. and, in the presentinvention, fucoidan prepared as such can be used. The use of seaweed andtrepang containing high amount of fucoidan are particularly preferred inthe present invention.

Examples of fucoidan of the present invention are fucoidan in a purifiedform and/or fucoidan-containing substances.

Examples of the fucoidan-containing substances are fucoidan-containingextracts.

The fucoidan-containing extracts include an extract from a raw materialderived from fucoidan-containing substances and a product prepared bytreating said extract where the extract containing a high amount offucoidan is preferred.

Examples of said fucoidan-containing extract such as an extract ofseaweed are extracts obtained by extracting the seaweed in the presenceof calcium salt. Calcium chloride may, for example, be used as thecalcium salt.

Examples of said seaweeds extract are soluble substances which areobtained by extracting the seaweed with an alkaline solution followed byadding a calcium salt to the extract. A sodium carbonate solution may,for example, be used as the alkaline solution while calcium chloridemay, for example, be used as the calcium salt.

Examples of said seaweed extracts are the soluble substances which areobtained by extracting the seaweed with an alkaline solution followed byacidifying the resulting extract. A sodium carbonate solution may, forexample, be used as the alkaline solution.

Temperature and time for extraction of raw materials derived fromfucoidan-containing substances may be selected from the range of 40-200°C. and 1-360 minutes, respectively and, usually, they may be selectedfrom the range of 50-130° C. and 10-180 minutes, respectively.

An example of the fucoidan which is used in the present invention is asubstance where the fucoidan content is increased in an aqueous extractof the raw material derived from the fucoidan-containing substance andwhere the content of algins is substantially reduced or removed. In sucha substance, it is possible to offer a material (1) having no propertiessuch as high viscosity and acid coagulation caused by algins andgelation due to multivalent metal ions which have affected food andbeverage, and (2) having no influence on the physical propertiesinherent to food and beverage.

Especially in the product prepared by treating the extract of seaweedwith active carbon, only the smell of seaweed is selectively removed andsaid product is particularly preferred for use as food and beveragewhere the seaweed smell is not required.

Fucoidan used in the present invention can be supplied in largequantities starting from edible fucoidan-containing substance such asedible seaweed and trepang and it has an extremely high safety.

There is no particular limitation for the food and beverage of thepresent invention and the examples are processed cereals [such asprocessed wheat flour products, processed starch products, processedpremix products, noodles, macaroni, bread, bean jams, soba (Japanesebuckwheat noodles), fu (Japanese wheat gluten bread), biifun (Chinesebean jelly sticks), harusame (Japanese bean jelly sticks) and packedmochi (rice cake)], processed fat/oil products [such as plastic fat/oil,oil for deep frying, salad oil, mayonnaise and dressing], processedsoybean products [such as tofu (soybean curd), miso (fermented soybeanpaste) and natto (fermented soybeans)], processed meat products [such asham, bacon, press ham and sausage], marine products [such as frozenground fish meat, kamaboko (boiled fish paste), chikuwa (another type ofboiled fish paste), hanpen (fish cake), satsuma-age (fried fish balls),tsumire (boiled fish paste), suji (fish muscle products), fish meat ham,fish meat sausage, dried bonito, processed fish egg products, cannedmarine products and tsukudani (fish boiled down in soy sauce)], milkproducts [such as raw milk, cream, yogurt, butter, cheese, condensedmilk, powdered milk and ice cream], processed vegetable/fruit products[such as pastes, jams, pickles, fruit beverages, vegetable beverages andmixed beverages], confectioneries [such as chocolate, biscuit, bun,cake, mochi-gashi (rice ball cake) and rice cracker], alcoholicbeverages [such as Japanese sake wine, Chinese wine, wine, whisky,shochu (Japanese distilled spirits), vodka, brandy, gin, ram, beer,alcoholic beverage for refreshment, fruit wine and liquors], tablebeverages [such as green tea, tea, oolong tea, coffee, beverages forrefreshment and lactic acid beverages], condiments [such as soy sauce,Wooster sauce, vinegar and mirin (sweet sake)], canned, bottled orpacked foods [gyumeshi (stewed beef with rice), kamameshi (boiled riceplaced in a small kettle-like container), sekihan (rice boiled with redbeans), curried rice and other retort foods)], semi-dried orconcentrated foods [such as lever paste and other spread, soup for sobaor udon and concentrated soup], dried foods [such as instant noodles,instant curry roux, instant coffee, powdery juice, powdery soup, instantmiso soup and retort foods, beverages and soup], frozen food [such asfrozen sukiyaki, chawan-mushi (pot-steamed hotch-potch), unagi-kabayaki(broiled eels), hamburger steak, shao-mai (Chinese steamed dumpling),gyoza (fried dumpling stuffed with minced pork, etc.), sticks and fruitcocktails], solid foods, liquid foods [such as soup], spices and otherprocessed agricultural, forest, stock raising and marine products.

There is no particular limitation for manufacturing the food andbeverage of the present invention but any cooking and processing meansand any of the manufacturing methods which have been usually conductedmay be used provided that the resulting food or beverage containsfucoidan. When an effective amount of apoptosis-inducing fucoidan isadded to food or beverage, it is possible to offer apoptosis-inducingfood or beverage.

In the case of cooking and processing, fucoidan may be added before,during or after the cooking/processing. Alternatively, cooked orprocessed thing or a material therefor may be added to fucoidan so thatfucoidan is diluted. In the manufacture of food or beverage, fucoidanmay be added during any of the steps, or food or beverage or materialthereof may be added to fucoidan so that fucoidan is diluted and iscontained in the food or beverage. The addition may be conducted at onetime or separately over several times. Thus, it is possible to easilymanufacture the novel food or beverage or the apoptosis-inducing food orbeverage having an apoptosis-inducing action containing an effectiveamount of fucoidan. In case any of the above-mentioned steps is applied,then the food or beverage where fucoidan is contained therein, addedthereto and/or diluted therein and the apoptosis-inducing food orbeverage where fucoidan is contained therein, added thereto and/ordiluted therein containing an effective amount of fucoidan may bedefined as the food or the beverage of the present invention.

The amount of fricoidan to be added to food was then investigated usinga model beverage as described below.

INVESTIGATING EXAMPLE 1

In order to conduct a model test beverage, a 0.01M lactic acid buffer ofpH 3.8 was prepared and then commercially available sucrose wasdissolved therein to make the sucrose concentration 5 w/v % whereupon asucrose solution was prepared. Fucoidan II of Example 1 was used and 0(control), 0.1, 0.5, 1, 5, 10, 20, 30, 40, 50, 100, 150 and 200 mg w/v %of it were added whereupon the sucrose solutions containing fucoidanwere prepared and were subjected to an organoleptic test for their feelupon drinking in terms of mellowness and smoothness on tongue, feel uponpassing through the throat and a balance of the taste. Numbers of thepanelists were 20 and the test was conducted by means of a five-pointevaluation method (where 5 stands for good while 1 stands for bad). Theresults are given in Table 1 in terms of average values.

TABLE 1 Organoleptic Test of the Model Beverages Amount of Feel BalanceTotal Fucoidan Touch on Tongue passing of Feel Added Mellow- Smooth- thethe on (mg w/v %) ness ness Throat Taste Eating 0 1.2 1.4 1.6 1.5 1.40.1 1.6 1.7 1.7 1.6 1.6 0.5 2.0 1.9 1.8 1.7 1.8 1 2.1 2.2 2.0 1.9 2.0 52.5 2.6 2.7 2.8 2.6 10 3.5 3.6 3.4 3.8 3.6 20 4.0 4.2 4.1 4.5 4.2 30 4.54.7 4.3 4.5 4.5 40 4.6 4.7 4.3 4.6 4.6 50 4.7 4.7 4.5 4.8 4.7 100 4.34.6 4.6 4.8 4.6 150 4.0 3.9 3.2 3.8 3.7 200 3.8 3.6 3.1 3.6 3.5

From Table 1, it is noted that, in view of feel on tongue, passing thethroat and balance of the taste in the organoleptic evaluations, aneffect is noted by addition of fucoidan, i.e. 0.1 mg w/v % or moreconcentrations.

INVESTIGATING EXAMPLE 2

To the model beverage of Example 1 containing 30 mg w/v % of fucoidan IIwas added alginic acid derived from seaweed in an amount of 0 (control),5, 10, 20, 30, 40, 60, 80, 100, 150 or 200 mg w/v % and the influence ofthe ratio of fucoidan to alginic acid existing therein on the feel oneating was tested by means of the same organoleptic test as inInvestigating Example 1. Average values of the results are given inTable 2.

TABLE 2 Influence of Fucoidan and Alginic Acid on OrganolepticEvaluations Al- Fuc- Feel on Tongue Feel Balance Total ginic Fuco- oi-Mel- on of Evaluation Acid idan dan low- Smooth- the the on (mg w/v %)Ratio* ness ness Throat Taste Eating 0 30 100  4.5 4.7 4.3 4.5 4.5 5 3085 4.5 4.6 4.2 4.5 4.4 10 30 75 4.3 4.5 4.2 4.5 4.4 20 30 60 4.2 4.4 4.14.5 4.3 30 30 50 4.2 4.4 4.1 4.4 4.3 40 30 43 4.0 4.1 3.7 3.9 3.9 60 3033 3.5 3.6 3.1 3.2 3.3 80 30 27 3.2 3.3 3.2 3.1 3.2 100 30 23 3.2 3.13.0 3.1 3.1 (remained a bit on tongue) 150 30 17 2.9 2.8 2.5 3.0 2.8(remained a bit on tongue) 200 30 13 2.7 2.6 2.3 2.6 2.6 (remained a biton tongue) *(Fucoidan)/(Alginic Acid + Fucoidan) (%)

It is noted from Table 2 that the effect on feel upon eating was highestin the model beverage when only fucoidan was added thereto and that,when the ratio of weight of fucoidan to that of alginic acid andfucoidan (hereinafter, referred to as a fucoidan ratio) becomes smaller,the evaluation of feel on eating becomes poor. In the organolepticevaluation data, the effect achieved by addition of 10 mg w/v % offucoidan was noted when the fucoidan ratio was 43% or more. The effectachieved by addition of 5 mg w/v % of fucoidan was noted when thefucoidan ratio was 13% or more while the cases where no remaining feelon tongue was noted were achieved when the ratio was 27% or more.

There is no particular limitation for the amount of fucoidan in food orbeverage of the present invention but the amount may be appropriatelyselected by taking the organoleptic and physiological activities intoconsideration. For example, its amount calculated as fucose per 100parts of food or beverage by a cysteine-sulfuric acid method is 0.001part or more and, in view of the organolepticity as food or of the tasteas beverage, in view of induction of apoptosis and also in view of thecost, said amount is preferably 0.005-10 parts or, more preferably,0.01-1.0 part. Incidentally, the term “part(s)” used in thisspecification stands for that/those by weight.

There is no particular limitation for the form of the food or beverageof the present invention so far as fucoidan of the present invention iscontained therein and said form includes that which can be orally takensuch as tablets, granules, capsules, gel and sol.

The food or beverage of the present invention contains an effectiveamount of fucoidan having a physiological activity and, due to anapoptosis-inducing action or the like of said fucoidan, it is a healthyor functional food or beverage having an effect of preventingcarcinogenesis or suppressing cancer. It is a food or beverage which isparticularly useful in maintaining stomach and intestine in a healthystate.

In the present invention, fucoidan is a polysaccharide containing fucosesulfate in a molecule and/or a degraded product thereof and there is noparticular limitation. Incidentally, the fucose-containingpolysaccharide derived from brown algae plants is usually calledfucoidan, fucoidin or fucan and, although several molecular speciesthereof have been known already, fucoidan of the present inventioncovers any and all of them.

Examples of a method for degradation of fucoidan are a chemicallydegrading method by treating it with an acid, etc., a physicallydegrading method by treating it with ultrasonic wave, etc., a method ofdegrading it with enzymes and a method of degrading it withmicroorganisms. In the present invention, any of the degraded fucoidanswhich contain sulfated fucose in the molecule and show anapoptosis-inducing action may be used.

In said fucoidan molecular species, there are a group where fucose is amain component and another group where several % of uronic acid iscontained and, as constituting saccharides, much amounts of fucose andmannose are contained therein. Hereinafter, a group where uronic acid isnot substantially contained therein will be referred to as fucoidan-Fwhile another where uronic acid is contained therein will be referred toas fucoidan-U and a mixture of them will be just referred to asfucoidan.

In the present invention, one of fucoidan-F and fucoidan-U may be usedsolely or both of them may be used jointly. Thus, the food or beveragewhere pure fticoidan-F or fucoidan-U is a main component is covered bythe present invention as well. Such a food or beverage containing anincreased amount of said fucoidans, particularly fticoidan-U, has apotent effect for promoting the health.

The extract derived from fucoidan-containing natural products accordingto the present invention contains fucoidan-U having the followingphysical and chemical properties which is prepared, for example, by amethod as shown in Example 4.

(1) component sugar: containing uronic acid; and

(2) being degraded by the endo-fucoidan-lyase produced by Flavobacteriumsp. SA-0082 (FERM BP-5402) to thereby form at least one of the compoundsselected from those represented by the following formulae (I), (II) and(III).

The extract derived from fucoidan-containing natural products accordingto the present invention contains fucoidan-F having the followingphysical and chemical properties which is prepared, for example, by amethod as shown in Example 5.

(1) component sugar: substantially being free from uronic acid; and

(2) substantially incapable of being degraded by the endo-fucoidan-lyaseproduced by Flavobacterium sp. SA-0082 (FERM BP-5402).

When fucoidan is treated with a fucoidan-degrading microorganism such asthe above-mentioned endo-fucoidan-lyase-productive Flavobacterium sp.SA-0082 (FERM BP-5402), a microbiologically degraded fucoidan can beprepared.

Further, when the above-mentioned fucoidan-U is treated with aendo-fucoidan-lyase such as that produced by Flavobacterium sp. SA-0082(FERM BP-5402), enzymatically degraded fucoidan-U can be prepared. Thesedegraded fucoidan and/or fucoidan-U can be easily fractionated accordingto their molecular weight. Food or beverage where those degradedfucoidans are contained therein, added thereto and/or diluted therewithis the food or beverage of the present invention as well.

In the manufacture of an extract derived from seaweed which is one ofthe examples of the fucoidan contained in the food or beverage of thepresent invention, the seaweed which is applicable is, for example,Rhodophyceae such as common laver (Porphyra tenera), Gelidiumcartiliagimeum and Gracilaria confervoides; Chlorophyceae such as Ulvalactuca; and Phaeophyceae such as Ecklonia cava, Eisenia arboria var.bicyclis, Nemacystus dicipiens, Hizikia fusiforme, Undaria pinnatifida,Kjellmaniella crassifolia and Laminaria japonica and, among them,seaweed containing high amount of fucoidan is particularly suitable inthe present invention.

With respect to the seaweed used in the present invention, the seaweedwhich contains high amount of fucoidan such as Phaeophyceae may, forexample, be directly dried, milled and extracted or the fresh one may befinely cut and extracted.

Any extracting method may be used so far as fucoidan can be efficientlyextracted.

The process for the manufacture of a seaweed extract may contain a stepof washing the seaweed with water, alcohol or aqueous alcohol.Extraction of seaweed may be conducted in the presence of alcohol aswell.

In the present invention, seaweed is extracted, for example, with asolution of calcium salt such as calcium chloride to prepare an extractof seaweed with a calcium chloride solution.

It is preferred that the extraction is conducted using 10-1,000 parts ofcalcium chloride solution to one part of dry seaweed and that theconcentration of calcium chloride is 25 mM or more.

When fresh seaweed is used, it is preferred to use 1-100 parts ofcalcium chloride solution to one part of the fresh seaweed and that theconcentration of calcium chloride is 25 mM or more.

It is preferred that the extraction is conducted within a range of50-130° C. and 10-180 minutes. Any extracting condition may be adoptedso far as the amount of algins in the extract is reduced wherebyfucoidan can be efficiently extracted.

After completion of the extraction of the seaweed with a calcium saltsolution, insoluble matters are removed. Method for the removal of theinsoluble matters may be selected from centrifugation, filtration, etc.Calcium salt in the solution wherefrom the insoluble matters are removedcan be removed, for example, by ultrafiltration or ion exchangingmethod. The solution wherefrom the calcium salt is removed can befurther treated with active carbon, ion exchanger, etc. so that theseaweed smell can be selectively eliminated.

In the present invention, seaweed is, for example, extracted with analkaline solution such as sodium carbonate solution to prepare anextract of the seaweed with sodium carbonate. Then calcium salt such ascalcium chloride is added to said extract and the resulting insolublematters are removed whereupon the soluble fractions of theseaweed-derived extract can be prepared.

It is preferred that the extraction is conducted using. 10-600 parts ofsodium carbonate solution to one part of the seaweed and that theconcentration of sodium carbonate is 0.1-5%.

When fresh seaweed is used, it is preferred that 1-60 parts of sodiumcarbonate solution is used to one part of the fresh seaweed and that theconcentration of sodium carbonate is 0.1-5%.

It is preferred that the extraction is conducted within a range of50-130° C. and 10-180 minutes. Any extracting condition may be adoptedprovided that fucoidan can be efficiently extracted in the extract.Incidentally, the term % used in this specification stands for that byweight.

After completion of extraction of the seaweed with a sodium carbonatesolution, calcium salt such as calcium chloride is added thereto and theresulting insoluble components are removed. Amount of calcium chlorideto be added is in such an extent that no more algins are precipitated byadding it to the sodium carbonate extract and any conditions will do sofar as the algins in the extract can be efficiently precipitated. Methodfor the removal of the resulting insoluble components may be selectedfrom centrifugation, filtration, etc. The salts such as sodium carbonateand calcium chloride in the solution wherefrom the insoluble componentsare removed can be removed by, for example, means of ultrafiltration andion exchanging. The solution wherefrom the salts are removed may befurther treated with active carbon, ion exchanger, etc. so that itsseaweed smell can be selectively eliminated.

In the present invention, the seaweed is, for example, extracted with analkaline solution such as sodium carbonate solution to prepare anextract of seaweed with sodium carbonate. After that, the extract isacidified with an acid such as diluted sulfuric acid and the resultinginsoluble components are removed whereupon a soluble substance of theseaweed-derived extract is prepared.

Extraction of the seaweed with a sodium carbonate solution is conductedaccording to a method which was mentioned already.

After completion of extraction of the seaweed with a sodium carbonatesolution, an acid solution such as diluted sulfuric acid is addedthereto and the resulting insoluble components are removed. Amount ofthe diluted sulfuric acid to be added is in such an extent that pH ofthe extract with sodium carbonate becomes 1-2.5 and any conditions willdo so far as the extract is made acidic whereby the algins in theextract can be efficiently precipitated. Method for the removal of theresulting insoluble components may be selected from centrifugation,filtration, etc. The salts in the solution wherefrom the insolublecomponents are removed can be eliminated by, for example, means ofultrafiltration and ion exchanging. The solution wherefrom the salts areremoved may, if necessary, be neutralized and, when the acidic orneutralized solution may be further treated with active carbon, ionexchanger, etc. so that its seaweed smell can be selectively eliminated.

Fucoidan of the present invention may be either liquid or solid. In themanufacture of solid fucoidan, known methods such as a spray-dryingmethod and a freeze-drying method may be suitably selected.

Usually, fucoidan is weak to acids and alkalis and, therefore, it is aptto be degraded into low molecular substances when an acidic or alkalinesolution is used. When heating temperature, heating time, pH, etc. areappropriately adjusted, it is possible to prepare a desired degradedproduct. It is possible to adjust the average molecular weight and themolecular weight distribution by, for example, means of gel filtration,treatment with membrane for fractionating the molecular weight, etc.Molecular weight and sugar component of fucoidan vary depending upon theharvest time of the material for fucoidan, the method for drying saidmaterial, the method for storing said material, etc. and also dependingupon heating conditions, pH conditions, etc. during the extractingprocess of fucoidan. For example, fucoidan is hydrolyzed by acid while,under an alkaline condition, it becomes to low molecular products as aresult of a β-elimination of uronic acid. Accordingly, with respect tofucoidan-U and fucoidan-F mentioned in this specification, the molecularweight and the molecular weight distribution given therefor are justexamples and can be easily changed by adjusting the treating conditionsfor fucoidan. For example, when fucoidan is heated at 100° C. for onehour under a weakly alkaline condition and when a molecular sievemembrane having a pore size of 300 is used for removal of the salt, itis possible to prepare fucoidan, fucoidan-U, fucoidan-F, etc. having amolecular weight distribution of from about 1,000 to about 10,000.Depending upon the conditions applied, fucoidan having any molecularweight and any molecular weight distribution can be prepared and such aproduct may be used as a fucoidan of the present invention.

When fucoidan and/or degraded product thereof in accordance with thepresent invention are/is added to a culture liquid of cancer cells,apoptosis is resulted in said cancer cells after one to several daysfrom the addition. Incidentally, they do not show toxicity to normalcells. Particularly, fucoidan derived from edible seaweed and/ordegraded product thereof have/has a high safety.

The present invention will be further illustrated by way of thefollowing examples although the present invention is never limited bythose examples.

EXAMPLE 1

(1). Kjellmaniella crassifolia was well dried and 20 kg of the dried onewas milled by a free disintegrator (mfd. by Nara Kikai Seisakusho).

Calcium chloride dihydrate (7.3 kg) (mfd. by Nippon Soda) was dissolvedin 900 liters of tap water and mixed with 20 kg of the milledKjellmaniella crassifolia. Steam was blown thereinto whereby the liquidtemperature was raised from 12° C. to 90° C. within 40 minutes, then thetemperature was kept at 90-95° C. for one hour with stirring and cooledto give 1,100 liters of cooled mixture.

This was subjected to a treatment with a solid-liquid separator (typeCNA; mfd. by Westfalier Separator) to separate the cooled mixture intosolid and liquid whereupon about 900 liters of a supernatant liquid wasprepared.

The supernatant liquid (360 liters) was concentrated to 20 liters usingan FE10-FC-FUS0382 (cut off molecular weight: 30,000) (mfd. by Daicel).To this was added 20 liters of tap water followed by concentrating to 20liters and such operations were repeated for five times to remove thesalt therefrom whereupon 25 liters of an extract solution derived fromseaweed containing high amount of fucoidan was prepared.

The extract solution had a pH of about 6.5, acidity of 0.06 ml, sugardegree of 0.8 Brix % and calcium concentration of 1,200 ppm.

Said solution (one liter) was freeze-dried to give 13 g of a driedproduct in which 65% of fucoidan and 33% of fucoidan-U were contained.

Content of fucoidan-U was measured by means of an HPLC using a standardfucoidan-U substance which was prepared in Example 4.

Incidentally, content of fucoidan was calculated by the followingsulfuric acid-cysteine method using an aqueous solution of the standardfucoidan (0.4 mg/ml) prepared in Example 5.

The testing solution (200 μl) was taken in a test tube, 900 μl of a 6:1mixture of concentrated sulfuric acid and water was added thereto undercooling with ice and the resulting mixture was well cooled and stirred.This was kept at about 20° C. for three minutes and heated in a boilingwater bath for ten minutes. After heating, it was cooled in ice waterand 20 μl of a 3% cysteine-hydrochloric acid was added followed bystirring (a colored section). As a control, 20 μl of water were addedthereto followed by stirring (a blank). After stirring, the mixture wasallowed to stand for one hour and the absorbances at 400 nm and 460 nmwere measured.

Amount of fucoidan in the tested solution was calculated by thefollowing formula.

Amount (mg/ml)=Test Solution [Colored Section (A₄₀₀-A₄₆₀)−Blank(A₄₀₀-A₄₆₀)]/Standard Solution [Colored Section (A₄₀₀-A₄₆₀)−Blank(A₄₀₀-A₄₆₀)]×diluting ratio×20.4

In the formula, A₄₀₀ is an absorbance at 400 nm while A₄₆₀ is that at460 nm.

Incidentally, pH was measured by a pH meter while acidity was expressedby the amount (ml) of 0.1N NaOH required for neutralizing 10 ml of thesample solution to pH 7.0. Sugar degree was measured by a Brixsaccharometer while concentration of calcium was measured by means ofatomic absorption spectrometry.

Then, to the extract solution derived from seaweed containing highamount of fucoidan was added 2% of active carbon (available as a foodadditive; Shirasagi Brand) and the mixture was treated for 30 minutes,roughly filtered and finally filtered using a 0.8 μm filter to prepare afiltrate (fucoidan I) passing through a filter. Then one half of thefiltrate was heated at 120° C. for 60 minutes with pressure to prepare athermally treated liquid (fucoidan II).

The filtrate passed through the filter was freeze-dried and 10 mg of thedried product was suspended in each 10 ml of 1% sodium carbonatesolution and 100 mM calcium chloride solution. In both solutions, thedried product was completely dissolved and no contamination with alginswas noted.

(2) Kjellmaniella crassifolia was well dried and 20 kg of the driedproduct was milled using a disintegrator (Fitz Mill; mfd. by HosokawaMicron).

Calcium chloride dihydrate (mfd. by Nippon Soda) (7.2 kg) was dissolvedin 400 liters of tap water followed by mixing with 20 kg of milledKjellmaniella crassifolia. Steam was blown into the mixture withstirring for 95 minutes whereby the liquid temperature was raised from28° C. to 95° C. and the mixture was kept at 95° C. for two hours withstirring and cooled to give 500 liters of cooled product.

The cooled product was subjected to a solid-liquid separation using asolid-liquid separator (mfd. by Tanabe Weltech) to prepare about 450liters of a supernatant liquid after the solid-liquid separation.

Said supernatant liquid was concentrated to 40 liters using anFE10-FC-FUS0382 (cut off molecular weight: 30,000) (mfd. by Daicel).After that, sterilized water by filtration was continuously addedthereto to make the flow rate 40-60 liters/hour and a desaltingtreatment was conducted therefor until electroconductivity became 1.0mS/cm² whereupon 40 liters of an extract solution derived from seaweedcontaining high amount of fucoidan was prepared.

Then, to said extract solution were added 0.4 kg of Celite #545 (mfd. byCelite) and 0.4 kg of Silica #600-S (mfd. by Chuo Silica) as thefiltering aids and the mixture was filtered using a compact filter (16stage×6 inches; filter paper: ADVANTEC #327) precoated with 0.1 kg ofCelite #545 and 0.1 kg of Silica #600-S.

The resulting filtrate was subjected to a continuous instant heatingtreatment using a plate heater (mfd. by Nichihan Seisakusho) at 98° C.for 60 seconds and cooled to prepare 46 liters of an extract solution(fucoidan V) derived from seaweed containing high amount of fucoidan.

Said extract solution had a pH of about 7, acidity of 0 ml, sugar degreeof 1.2 Brix %, calcium concentration of 920 ppm, solid content of 1.2%and fucoidan content of 17 mg/ml. Neither alginic acid nor iodine wasdetected.

The content of alginic acid was measured by means of an HPLC using acommercially available alginic acid as a standard substance.

The content of fucoidan was calculated by means of a sulfuricacid-cysteine method using an aqueous solution (0.4 mg/ml) of a standardfucoidan substance prepared in Example 5 as a standard solution.

Electroconductivity was measured by an electroconductivity meter. Iodinewas separated by bromine and was titrated with sodium thiosulfate. Solidcontent was determined from the dry weight after a treatment withcentrifugal evaporator (at 60° C. for 18 hours).

(3) Kjellmaniella crassifolia was well dried and 20 kg of the driedproduct was milled using a disintegrator (Fitz Mill; mfd. by HosokawaMicron).

Calcium chloride dihydrate (mfd. by Nippon Soda) (7.3 kg) was dissolvedin 400 liters of tap water followed by mixing with 20 kg of milledKjellmaniella crassifolia. Steam was blown into the mixture withstirring for 95 minutes whereby the liquid temperature was raised from28° C. to 95° C. and the mixture was kept at 95° C. for two hours withstirring and cooled to give 500 liters of cooled product.

The cooled product was subjected to a solid-liquid separation using asolid-liquid separator (mfd. by Tanabe Weltech) to prepare about 450liters of supernatant liquid after the solid-liquid separation.

Said supernatant liquid was concentrated to 40 liters using anFE10-FC-FUS0382 (cut off molecular weight: 30,000) (mfd. by Daicel).After that, sterilized water by filtration was continuously addedthereto to make the flow rate 30 liters/hour and a desalting treatmentwas conducted therefor until electroconductivity became 1.0 mS/cm²whereupon 34 liters of an extract solution derived from seaweedcontaining high amount of fucoidan were prepared.

After that, 1.1 kg of pectin (Pomocin Pectin LM-13CG; mfd. by Hercules)was added to 20 liters of tap water, steam was blown thereinto so thatthe liquid temperature was raised from 28° C. to 120° C. for 35 minutesand the mixture was kept at 120° C. for five hours with stirring andcooled to prepare 28 liters of a cooled product.

Then 34 liters of the extract solution derived from seaweed containinghigh amount of fucoidan were mixed with 28 liters of heat-treated pectinand the mixture was adjusted to pH 3.5 with citric anhydride (mfd. byFuso Kagaku). After that, 0.9 kg of Celite #545 (mfd. by Celite) and 0.8kg of Silica #600-S (mfd. by Chuo Silica) were added as filtering aidsthereto and the mixture was filtered using a compact filter (16 stage×6inches; filter paper: ADVANTEC #327) precoated with 0.2 kg of Celite#545 and 0.2 kg of Silica #600-S.

To the resulting filtrate was added 18 liters of pure water and theresulting mixture was subjected to a continuous instant heatingtreatment using a plate heater (mfd. by Nichihan Seisakusho) at 98° C.for 60 seconds and cooled to prepare 80 liters of an extract solution(fucoidan VI) derived from seaweed containing high amount of fucoidan.

Said extract solution had a pH of about 3.5, acidity of 1.7 ml, sugardegree of 2.1 Brix %, calcium concentration of 710 ppm, solid content of2.0%, fucoidan content of 13 mg/ml and fucoidan-U content of 6.6 mg/ml.Neither alginic acid nor iodine was detected.

One liter of said solution was freeze-dried to give 20 g of a driedproduct.

EXAMPLE 2

Fresh Laminaria japonica was finely cut, 200 g of the resulting finelycut one were suspended in 600 ml of a 1% aqueous solution of sodiumcarbonate, the suspension was heated at 60° C. for one hour and waterwas added thereto to make the whole volume one liter. This solution wascentrifuged, the supernatant liquid was collected and water was addedthereto to make the whole volume four liters.

To this solution was added 500 mM of calcium chloride until no moreprecipitate was formed and the resulting precipitate was removed by afilter paper. The resulting filtrate was concentrated and desalted witha Microacilyzer to prepare 3.6 liters of a desalted solution. One halfof it was freeze-dried to give 2.1 g of an extract fraction (fucoidanIII) derived from seaweed containing high amount of fucoidan. Anotherhalf of the desalted solution was treated with 2% active carbon followedby heating with pressure at 120° C. for 60 minutes to prepare aheat-treated solution (fucoidan IV).

The above freeze-dried product (10 mg) was suspended in each 10 ml of a1% sodium carbonate solution and a 100 mM calcium chloride solution. Inboth solutions, said fraction was completely dissolved and nocontamination of algins was noted.

EXAMPLE 3

Apoptosis-Inducing Activity of the Extracts Derived from SeaweedContaining High Amount of Fucoidan Prepared in Examples 1 and 2.

Human promyelocytic leukemia cells HL-60 (ATCC CCL-240) which wasincubated at 37° C. in an RPMI 1640 medium (mfd. by Gibco) containing10% of fetal calf serum (JRH Bioscience) treated at 56° C. for 30minutes was suspended in an ASF 104 medium (mfd. by Ajinomoto) to make asuspension containing 5×10⁵ cells/9 ml. To 9.0 ml of this suspension wasadded 1 ml of each of the solutions of extract (fucoidan I and III)derived from seaweed containing high amount of fucoidan prepared inExamples 1 and 2 [prepared by dissolving each of the fucoidans in 50 mMHEPES buffer (pH 7.2) containing 100 mM of sodium chloride to make 5mg/ml concentration followed by sterilizing at 121° C. for 20 minutes]and each mixture was incubated at 37° C. for 18 hours in the presence of5% carbon dioxide. The incubated cells were centrifuged to separate froma supernatant liquid. The resulting cells were suspended in 20 μl of a50 mM Tris hydrochloride buffer (pH: 7.8) containing 10 mM ofethylenediamine tetraacetate and 0.5% of sodium lauroyl sarcosinate,then 1 μl of ribonuclease A (10 mg/ml) (mfd. by Sigma) was added theretofollowed by treating at 50° C. for 30 minutes and 1 μl of proteinaseK(10 mg/ml) was added thereto followed by treating at 50° C. for onehour. The cells after the treatment were used as a sample and subjectedto an electrophoresis using a 2% agarose gel under constant voltage of100 volts. The gel was dipped in ethidium bromide for 30 minutes andthen the state of DNA in the gel was checked using a transilluminatorwhereupon a DNA ladder which was specific to apoptosis was confirmed.

It was now apparent from this result that apoptosis was induced in HL-60cells by the extracts derived from seaweed containing high concentrationof fucoidan prepared in Examples 1 and 2.

Further, each of said extracts (fucoidan II and fucoidan IV) derivedfrom seaweed containing high amount of fucoidan prepared in Examples 1and 2 was dissolved in a physiological saline solution to make a 5 mg/mlsolution followed by sterilizing at 121° C. for 20 minutes and theresulting solution was used as a test solution for measuring theapoptosis-inducing activity according to the above-mentioned methodwhereupon the same effect was confirmed for each of the test solutions.

After that, human promyelocytic leukemia cells HL-60 which was incubatedat 37° C. in an RPMI 1640 medium (mfd. by Gibco) containing 10% of fetalcalf serum (JRH Bioscience) treated at 56° C. for 30 minutes wassuspended in an ASF 104 medium (mfd. by Ajinomoto) to make a suspensioncontaining 2.5×1 cells/4.5 ml. To 4.5 ml of this suspension was added0.5 ml of each of the solutions of extract (fucoidan I and III) derivedfrom seaweed containing high amount of fucoidan prepared in Examples 1and 2 [prepared by dissolving each of the fucoidans in 50 mM HEPESbuffer (pH 7.2) containing 100 nM of sodium chloride to make 2 mg/ml, 5mg/ml and 10 mg/ml concentrations followed by sterilizing at 121° C. for20 minutes] and each of the mixtures was incubated at 37° C. in thepresence of 5% carbon dioxide. After 24 hours and 44 hours from theinitiation of the incubation, each 0.5 ml of the culture liquid wastaken out as a sample and the viable cell numbers in the medium werecounted by a method mentioned in “Techniques in Tissue Culture” (secondedition) (published by Asakura Shuppan; edited by Japan Tissue CultureSociety; published on Nov. 1, 1990; pages 26-28). Thus, the counting wasconducted by a method where the cells were dyed with Trypan Blue on ahemocytometer.

The results are shown in FIGS. 2-4. FIG. 2 shows proliferation of thecells in the presence of 0.2 mg/ml of each fucoidan where the ordinateshows the viable cell numbers per ml of the culture liquid (×10⁴/ml;this will be applied to the descriptions hereinafter as well) while theabscissa shows incubation time (hours). FIG. 3 shows proliferation ofthe cells in the presence of 0.5 mg/ml of each fucoidan where theordinate shows the viable cell numbers per ml of the culture liquidwhile the abscissa shows incubation time (hours). FIG. 4 showsproliferation of the cells in the presence of 1 mg/ml of each fucoidanwhere the ordinate shows the viable cell numbers per ml of the cultureliquid while the abscissa shows incubation time (hours). In each ofFIGS. 2-4, an open triangle stands for the viable cell numbers at eachof the incubation time in the presence of an extract (fucoidan I)derived from seaweed containing high amount of fucoidan prepared inExample 1 while an open square stands for the viable cell numbers ateach of the incubation time in the presence of an extract (fucoidan III)derived from seaweed containing high amount of fucoidan prepared inExample 2. Incidentally, in the control where no sample was added, theviable cell numbers in the culture liquid were 1.5×10⁵/ml and 3.1×10⁵/mlafter incubations for 24 and 44 hours, respectively.

As shown in FIGS. 2-4, the extract derived from seaweed containing highamount of fucoidan was capable of killing the HL-60 cells by means ofapoptosis within two days provided that its concentration in the mediumwas at least 200 μg g/ml.

Further, each of said extracts (fucoidan II, fucoidan IV, fucoidan V andfucoidan VI) derived from seaweed containing high amount of fucoidanprepared in Examples 1 and 2 was dissolved in a physiological salinesolution to make 2-10 mg/ml solutions followed by sterilizing at 121° C.for 20 minutes. The resulting solutions were used as test solutions formeasuring the apoptosis-inducing activity according to theabove-mentioned method whereupon the same effect was confirmed for eachof the test solutions.

EXAMPLE 4

Preparation of Fucoidan-U.

Kjellmaniella crassifolia was well dried, 2 kg of the dried one wasmilled by a free disintegrator (mfd. by Nara Kikai Seisakusho), theresulting dried powder was suspended in nine liters of 80% ethanol andthe suspension was heated at 80° C. for two hours. After that, this wasfiltered through a filter paper to give a residue. The residue wassubjected to the above mentioned operations of washing with ethanol andfiltration thereafter and said operations were repeated for three timeswhereupon an ethanol-washed residue was obtained. This was suspended in36 liters of 0.2M calcium acetate solution, heated at 95° C. for twohours and filtered. The residue was washed with four liters of a 0.2Mcalcium acetate solution to give 36 liters of a fucoidan extract ofKjellmaniella crassifolia.

The above filtrate was concentrated to two liters by an ultrafiltraterequipped with an ultrafiltration membrane having an exclusion molecularweight of 100,000, salt was added thereto to make its finalconcentration 1.5M and then 5% cetyl pyridinium chloride was addedthereto until no more precipitate was formed. The precipitate obtainedthereby was removed by centrifugation. The resulting supernatant liquidwas concentrated to one liter by means of ultrafiltration, four litersof ethanol was added thereto and the resulting precipitate was collectedby means of centrifugation. To this precipitate was added 100 ml of a 4Maqueous sodium chloride solution, the mixture was well stirred, ethanolwas added to make its concentration 80% and the mixture was stirred andcentrifuged to give a precipitate. The precipitate was suspended in 80%ethanol followed by subjecting to centrifugation and such operationswere repeated until the absorption in the supernatant liquid at 260 nmdisappeared. The resulting precipitate was dissolved in two liters of a2M aqueous solution of sodium chloride, the resulting insoluble matterswere removed by means of centrifugation, 50 ml of DEAE-Cellofine A-800(mfd. by Seikagaku Kogyo) was added thereto, the mixture was stirred andthe resin added was removed by filtration. The filtrate was treated witha column of DEAE-Cellofine A-800 equilibrated with a 2M aqueous solutionof sodium chloride, the nonadsorbed matters were subjected toultrafiltration by an ultrafiltratet equipped with a holofiber which wasable to exclude the molecular weight of 100,000 and less whereuponcoloring substances and salt were completely removed and then theinsoluble matters were removed by means of centrifugation and filtrationfollowed by freeze-drying to prepare fucoidan-U. Weight of thefreeze-dried fucoidan-U was 15 g.

FIG. 1 shows the precipitate forming ability of this fucoidan-U andfucoidan-F which was prepared in the following Example 5 in variousconcentrations of sodium chloride in the presence of an excessive amountof cetyl pyridinium chloride.

In FIG. 1, the ordinate refers to the precipitation ratio (%) while theabscissa refers to the concentration (M) of sodium chloride. The solidline with open circles stands for the precipitation ratio of fucoidan-Uof the present invention at various sodium chloride concentrations (M)while the dotted line with open triangles stands for the precipitationratio of the fucoidan-F of the present invention at various sodiumchloride concentrations (M).

The precipitation ratios are determined at a solution temperature of 37°C. in the following manner.

Fucoidan-U and fucoidan-F are each dissolved in water and in a 4 M ofsodium chloride solution at a concentration of 2% each. Then thesesolutions are mixed at various ratios to thereby give 125 μl portions offucoidan-U and fucoidan-F solutions having various sodium chlorideconcentrations. Next, cetylpyridinium chloride is dissolved in water and4 M of sodium chloride at a concentration of 2.5% and the obtainedsolutions are mixed at various ratios to thereby give 1.25% solutions ofcetylpyridinium chloride with various sodium chloride concentrations.

3.2 times by volume as much the 1.25% solution of cetylpyridiniumchloride is needed to completely precipitate fucoidan-U and fucoidan-Feach dissolved in water at a concentration of 2%. To 125 μl portions of2% solutions of fucoidan-U and fucoidan-F with various sodium chlorideconcentrations are added 400 μl portions of cetylpyridinium chloridesolutions with various sodium chloride concentrations. After thoroughlystirring and allowing to stand for 30 minutes, each mixture iscentrifuged and the sugar content of the supernatant is determined bythe phenol-sulfuric acid method [Analytical Chemistry, 28, 350 (1956)]followed by the calculation of the precipitation ratio of each fucoidanat each sodium chloride concentration.

The molecular weight of fucoidan-U thus obtained is determined by thegel filtration method with the use of Sephacryl S-500. As a result, itshows a molecular weight distribution around about 190,000.

Next, the components of fucoidan-U are analyzed.

First, the fucose content is determined in accordance with the methoddescribed in Journal of Biological Chemistry, 175, 595 (1948).

Next, the dry preparation of fucoidan-U thus obtained is dissolved in 1N hydrochloric acid to give a concentration of 0.5% and treated at 110°C. for two hours to thereby hydrolyze into constituting sugars.Subsequently, the reducing ends of the monosaccharides obtained by thehydrolysis are pyridyl-(2)-aminated (PA) by using GlycoTAG and GlycoTAGReagent Kits (each mfd. by Takara Shuzo) and the composition ratio ofthe constituting sugars is analyzed by an HPLC.

Next, the content of uronic acid is determined in accordance with themethod described in Analytical Biochemistry, 4, 330 (1962).

Subsequently, the content of sulfuric acid is determined in accordancewith the method described in Biochemical Journal, 84, 106 (1962).

As a result, it is found out that the constituting sugars of fucoidan-Uare fucose, mannose, galactose, glucose, rhamnose, xylose and uronicacid and no other neutral sugar is substantially contained therein. Thecomposition ratio by mol of the major components is as follows;fucose:mannose:galactose:uronic acid:sulfate group=about 10:7:4:5:20.

Structure of fucoidan-U was determined as follows.

Degradation of fucoidan-U with degrading enzyme capable of degradingfucoidan-U and purification of the degradation product:

The purified fucoidan-U is treated with an endo-fucoidan-lyase as willbe described hereinafter and the degradation products are purified.

Namely, 16 ml of a 1% solution of fucoidan-U, 12 ml of a 50 mM phosphatebuffer (pH 8.0), 4 ml of a 4M solution of sodium chloride and 8 ml of a32 mU/ml solution of the endo-fucoidan-lyase are mixed together and madeto react at 25° C. for 48 hours. It is confirmed that the absorbance ofthe reaction mixture at 230 nm is elevated as the reaction proceeds,thus proving that the degradation of fucoidan-U with this enzyme is inprogress. After desalting with a Micro Acilyzer G3 (mfd. by AsahiChemical Industry), the reaction mixture is separated into threefractions (a), (b) and (c) with a DEAE-Sepharose FF.

The above-mentioned endo-fucoidan-lyase is prepared in the followingmanner.

The strain to be used in the production of this enzyme may be anarbitrary one, so long as it is capable of producing theendo-fucoidan-lyase. As a particular example thereof, citation can bemade of Flavobacterium sp. SA-0082 (FERM BP-5402).

This strain was isolated by the present inventors from seawater inAomori. It is indicated as Flavobacterium sp. SA-0082 and has beendeposited at National Institute of Bioscience and Human-Technology,Agency of Industrial Science and Technology (1-3, Higashi 1-chome,Tsukuba, Ibaragi, 305 Japan) under the accession number FERM P-14872since Mar. 29, 1995 and deposited at National Institute of Bioscienceand Human-Technology as described above under the accession number FERMBP-5402 (transfer to international deposition being requested on Feb.15, 1996).

The nutrients to be added to the medium for cultivating this strain maybe arbitrary ones so long as the strain employed can utilize them so asto produce the endo-fucoidan-lyase. Appropriate examples of the carbonsource include fucoidan, marine algae powder, alginic acid, fucose,glucose, mannitol, glycerol, saccharose, maltose, lactose and starchwhile appropriate examples of the nitrogen source include yeast extract,peptone, casamino acids, corn steep liquor, meat extract, defattedsoybean, ammonium sulfate and ammonium chloride. The medium may furthercontain inorganic matters and metal salts such as sodium salts,phosphates, potassium salts, magnesium salts and zinc salts.

The yield of the endo-fucoidan-lyase produced by cultivating the strainvaries depending on the cultivation conditions. In general, it ispreferable that the cultivation temperature ranges from 15 to 30° C. andthe pH value of the medium ranges from 5 to 9. The yield of theendo-fucoidan-lyase attains the maximum by cultivating the strain underaeration and agitation for 5 to 72 hours. As a matter of course, thecultivation conditions are appropriately selected depending on thestrain employed, the medium composition, etc. so as to achieve themaximum yield.

The endo-fucoidan-lyase is contained in both of the cells and theculture supernatant.

The above-mentioned Flavobacterium sp. SA-0082 is cultivated in anappropriate medium and the cells are harvested and disrupted by meanscommonly employed for disrupting cells such as ultrasonication. Thus acell-free extract can be obtained.

Subsequently, the extract is purified by a purification procedurecommonly employed in the art to thereby give a purified enzymepreparation. For example, the purification may be effected by saltingout, ion exchange chromatography, hydrophobic bond columnchromatography, gel filtration or the like to thereby give the purifiedendo-fucoidan-lyase free from any other endo-fucoidan-lyase.

The culture supernatant obtained by eliminating the cells from theabove-mentioned culture medium also contains a large amount of thisenzyme (extracellular enzyme) which can be purified by the same means asthose employed for purifying the intracellular enzyme.

Now an example of the purification of the endo-fucoidan-lyase will begiven.

Flavobacterium sp. SA-0082 (FERM BP-5402) is inoculated into 600 ml of amedium comprising an artificial seawater (pH 7.5, mfd. by JamarinLaboratory) containing 0.25% of glucose, 1.0% of peptone and 0.05% ofyeast extract which has been pipetted into a two-liter Erlenmeyer flaskand sterilized at 120° C. for 20 minutes. Then the strain is cultivatedtherein at 24° C. for 24 hours to thereby give a seed culture. Into athirty-liter jar fermenter is fed 20 liters of a medium comprising anartificial seawater (pH 7.5, mfd. by Jamarin Laboratory) containing0.25% of glucose, 1.0% of peptone, 0.05% of yeast extract and 0.01% of adefoaming agent (KM70 mfd. by Shin-Etsu Chemical Co., Ltd.) andsterilized at 120° C. for 20 minutes. After cooling, the medium isinoculated with 600 ml of the above-mentioned seed culture, which isthen cultivated therein at 24° C. for 24 hours under aerating at a rateof 10 liters/min and agitating at 125 rpm. After the completion of thecultivation, the culture medium is centrifuged to thereby collect thecells.

These cells are suspended in a 20 mM acetate phosphate buffer (pH 7.5)containing 200 mM of sodium chloride, disrupted by ultrasonication andcentrifuged to thereby give a cell extract. The endo-fucoidan-lyase inthis cell extract shows an activity of 5 mU/ml of the medium.

To this extract is added ammonium sulfate so as to establish 90%saturation finally. After dissolving by stirring, the mixture iscentrifuged and the precipitate is suspended in the same buffer as theabove-mentioned one in which the cells are suspended. Then thesuspension is thoroughly dialyzed against a 20 mM acetate phosphatebuffer (pH 7.5) containing 50 mM of sodium chloride. After eliminatingthe precipitate formed by the dialysis by centrifugation, it is absorbedby a DEAE-Sepharose FF column which has been equilibrated with a 20 mMacetate-phosphate buffer (pH 7.5) containing 50 m of sodium chloride.Then the adsorbed matter is well washed with the same buffer anddeveloped by a linear gradient elution with sodium chloride of 50 mM to600 mM. The active fractions are combined and sodium chloride is addedthereto so as to give a final concentration of 4 M. Next, it is adsorbedby Phenyl Sepharose CL-4B column which has been equilibrated with a 20mM phosphate buffer (pH 8.0) containing 4 M of sodium chloride. Then theadsorbed matter is well washed with the same buffer and developed by alinear gradient elution with sodium chloride of 4 M to 1 M. The activefractions are combined and concentrated with an ultrafiltrater. Next, itis subjected to gel filtration with the use of Sephacryl S-300 which hasbeen equilibrated with a 10 mM phosphate buffer containing 50 mM ofsodium chloride. The active fractions are combined. The molecular weightof the enzyme determined from the retention time in Sephacryl S-300 isabout 460,000. Next, the active fraction is dialyzed against a 10 mMphosphate buffer (pH 7) containing 250 mM of sodium chloride. The enzymesolution is adsorbed by a Mono Q HIR5/5 column which has beenequilibrated with a 10 mM phosphate buffer (pH 7) containing 250 mM ofsodium chloride. The adsorbed matter is well washed with the same bufferand developed by a linear gradient elution with sodium chloride of 250mM to 450 mM. The active fractions are combined to thereby give thepurified enzyme. Table 3 summarizes the above-mentioned purificationsteps.

TABLE 3 Total Total Specific protein activity activity Yield Step (mg)(mU) (mU/mg) (%) Cell extract 61,900 101,000  1.63 100 Ammonium sulfate-33,800 88,600 2.62 87.7 precipitation DEAE-Sepharose FF 2,190 40,40018.4 40.0 Phenyl Sepharose 48.2 29,000 601 28.7 CL-4B Sephacryl S-3007.24 19,600 2,710 19.4 Mono Q 0.824 15,000 18,200 14.9

The activity of this enzyme is determined in the following manner.

Fifty μl of a 2.5% solution of fucoidan originating in Kjellmaniellacrassifolia, 10 μl of this enzyme and 60 μl of a 83 mM phosphate buffer(pH 7.5) containing 667 mM of sodium chloride are mixed together andmade to react at 37° C. for 3 hours. Then 105 μl of the reaction mixtureis mixed with 2 ml of water under stirring and the absorbance (AT) ismeasured at 230 nm. As controls, use is made of a reaction mixtureprepared by the same method but substituting the enzyme by theabove-mentioned buffer alone employed for dissolving the enzyme andanother reaction mixture prepared by the same method but substitutingthe fucoidan solution by water alone and the absorbances (AB1 and AB2)thereof are also measured.

The amount of the enzyme by which one μmol of the glycoside bondsbetween mannose and uronic acid can be eliminatively cleaved in oneminute is taken as one unit (U). The bonds thus cleaved are determinedby taking the millimolar molecular extinction coefficient of theunsaturated uronic acid formed in the elimination reaction as 5.5. Theactivity of the enzyme is determined in accordance with the followingequation:

Activity (U/ml)=(AT−AB1−AB2)×2.105×120/(5.5×105×0.01×180);

2.105: volume (ml) of the sample the absorbance of which is to bemeasured;

120: volume (μl) of the enzyme reaction mixture;

5.5: millimolar molecular extinction coefficient (/mM) of unsaturateduronic acid at 230 nm;

105: volume (μl) of the reaction mixture employed for dilution;

0.01: volume (ml) of the enzyme; and

180: reaction time (min).

The protein is determined by measuring the absorbance of the enzymesolution at 280 nm and calculated by taking the absorbance of the 1mg/ml protein solution as 1.0.

The fucoidan originating from Kjellmaniella crassifolia employed as thesubstrate is prepared in the following manner.

Dry Kjellmaniella crassifolia is ground with a free mill Model M-2 (mfd.by Nara Kikai Seisakusho) and treated in 10 times as much 85% methanolat 70° C. for 2 hours. Then it is filtrated and the residue is furthertreated in 10 times as much methanol at 70° C. for 2 hours. Afterfiltrating, 20 times as much water is added to the residue. Then themixture is treated at 100° C. for 3 hours and filtrated to thereby givean extract. The salt concentration of the extract is adjusted to thesame level as that of a 400 mM solution of sodium chloride. Thencetylpyridinium chloride is added thereto until no more precipitation isformed. After centrifuging, the precipitate is thoroughly washed withethanol to thereby completely eliminate the cetylpyridinium chloride:Next, it is subjected to desalting and the removal of low-molecularweight substances by using an ultrafiltrater (exclusion molecular weightof ultrafiltration membrane: 100,000, mfd. by Amicon). The precipitatethus formed is eliminated by centrifugation. The supernatant isfreeze-dried to thereby give purified Kjellmaniella crassifoliafucoidan.

Analysis of the structure of enzyme reaction products:

The above-mentioned endo-fucoidan-lyase is an enzyme which eliminativelydegrades the α 1→4 bond between D-mannose and D-glucuronic acid incomplex polysaccharide. When fucoidan-U obtained above is treated withthis enzyme, oligosaccharides having the structures represented by thealready-mentioned formulae (I), (II) and (III) are formed.

A portion of each of the above-mentioned three fractions (a), (b) and(c) separated and purified by DEAE-Sepharose FF is pyridyl-(2)-aminated(PA) at the reducing end by using GlycoTAG and GlycoTAG Reagent Kits(both mfd by Takara Shuzo) to thereby give PA saccharides (PA-a), (PA-b)and (PA-c). Those (PA-a), (PA-b) and (PA-c) were analyzed by means ofHPLC and the difference from the PA products of three oligosaccharidesrepresented by the formulae (I), (II) and (III) was investigated.

Incidentally, conditions of the HPLC were as follows.

(i) HPLC analysis by using molecular weight fractionation column.

apparatus: Model L-6200 (mfd. by Hitachi, Ltd.);

column: SHODEX SB-803 (4.6×250 mm, mfd. by Showa Denko);

eluent: 0.2 M sodium chloride:dimethyl sulfoxide=9:1;

detection: Fluorometric Detector F-1150 (mfd. by Hitachi, Ltd.),excitation wavelength: 320 m, fluorescent wavelength: 400 nm;

flow rate: 1 ml/min.; and

column temperature: 50° C.

(ii) HPLC analysis with the use of reversed phase column.

apparatus: Model L-6200 (mfd. by Hitachi, Ltd.);

column: L-column (4.6×250 mm, mfd. by Kagaku Yakuhin Kensa Kyokai);

eluent: 50 mM acetic acid-triethylamine (pH 5.5);

detectin: Fluorometric Detector F-1150 (mfd. by Hitachi, Ltd.),excitation wavelength: 320 nm, fluorescent wavelength: 400 rim;

flow rate: 1 ml/min; and

column temperature: 40° C.

As a result of the above-mentioned two HPLC analyses, each of the threeoligosaccharides obtained by degrading fucoidan-U with the aboveendo-fucoidan-lyase was identical with the three oligosaccharidesrepresented by the above formulae (I), (II) and (III), respectively.

Therefore, the compound (a) has a structure wherein unsaturatedD-glucuronic acid and L-fucose having a sulfate group bonded thereto arebonded to D-mannose which is the reducing end; the compound (b) has astructure wherein unsaturated D-glucuronic acid and L-fucose having twosulfate groups bonded thereto are bonded to D-mannose which is thereducing end and has a sulfate group bonded thereto; and the compound(c) has a structure wherein D-glucuronic acid and L-fucose having asulfate group bonded thereto are bonded to D-mannose which is thereducing end, and to this D-glucuronic acid is bonded D-mannose and, inturn, to this D-mannose are further bonded unsaturated D-glucuronic acidand L-fucose having a sulfate group bonded thereto.

As discussed above, the obtained fucoidan-U has a structure whereinD-glucuronic acid and D-mannose are alternately bonded to each other andL-fucose is bonded to at least one D-mannose.

Also, it has a partial structure represented by the following generalformula (IV) wherein at least one of alcoholic hydroxyl groups has beensulfated and n stands for an integer of 1 or more.

Thus, when this fucoidan-U is treated with the above-mentionedendo-fucoidan-lyase, the oligosaccharides represented by thealready-mentioned formulae (I), (II) and (III) were produced.

Specific rotation of this freeze-dried fucoidan-U was measured by ahigh-speed and highly-sensitive polarimeter SEPA-300 (mfd. by Horiba,Ltd.) and found to be −53.6°.

EXAMPLE 5

(1) Preparation of standard fucoidan substance, fucoidan-F and fucoidanU.

Two kg of thoroughly dried Kjellmaniella crassifolia was ground with afree mill (mfd. by Nara Kikai Seisakusho). The dry powder thus obtainedwas suspended in 9 liters of 80% ethanol and treated at 80° C. for 2hours. Then the mixture was filtrated through a filter paper to therebygive the residue. This residue was repeatedly washed with ethanol andfiltered thrice in the same manner as described above to thereby givethe residue after ethanol-washing. This residue was suspended in 36liters of a 0.2 M solution of calcium acetate, treated at 95° C. for 2hours and filtrated. The residue was washed with 4 liters of a 0.2 Msolution of calcium acetate to thereby give 36 liters of an extractcontaining fucoidan of Kjellmaniella crassifolia. To the filtrate thusobtained was added 5% cetylpyridinium chloride until no precipitateswere formed any more. Then the precipitates were collected bycentrifugation and washed by suspending them in 3 liters of a 0.4 Maqueous solution of sodium chloride followed by centrifugation. Afterrepeating this washing procedure thrice, 1 liter of 4 M aqueous solutionof sodium chloride was added to the precipitate and the mixture wasstirred. Then ethanol was added thereto so as to give a concentration of80% and the mixture was stirred and centrifuged to thereby give theprecipitate. The obtained precipitate was suspended in 80% ethanol andcentrifuged. These procedures were repeated until the absorbance of thesupernatant at 260 nm reached zero. The precipitate was dissolved in 3liters of a 2 M aqueous solution of sodium chloride. After removing theinsoluble matters by centrifugation, 100 ml of DEAE-Cellulofine A-800(mfd. by Seikagaku Kogyo) was added thereto and stirred. Next, the resinadded above was removed by filtration. The filtrate was fed into aDEAE-Cellulofine A-800 column equilibrated with a 2 M aqueous solutionof sodium chloride and the unadsorbed fraction was subjected toultrafiltration by using an ultrafiltrater provided with a hollow fiberof exclusion molecular weight of 100,000 or less to thereby completelyeliminate the coloring matters and sodium chloride. Next, the insolublematters were eliminated by centrifugation and filtration followed byfreeze-drying to prepare a standard fucoidan substance.

Weight of said standard fucoidan substance was 90 g.

Seven grams of this freeze-dried standard fucoidan substance was weighedout and dissolved in 0.2 M calcium chloride. Then, 4,000 ml of aDEAE-Sepharose FF column was equilibrated with 0.2 M calcium chloride.The standard fucoidan substance dissolved in the 0.2 M calcium chloridewas fed into the DEAE-Sepharose FF column and thoroughly washed with 0.2M calcium chloride. Next, it was developed by linear gradient elutionwith sodium chloride of 0 to 4 M. Among the fractions thus obtained,those having sodium chloride concentration of 0.05 to 0.8 M werecombined, desalted by dialysis and then freeze-dried. Thus 2.1 g offucoidan-U substantially separated from fucoidan-F was obtained.

Among the fraction eluted above, those having sodium chlorideconcentration of 0.9 to 1.5 M were combined, desalted by dialysis andthen freeze-dried. Thus, 4.7 g of fucoidan-F substantially separatedfrom fucoidan-U was obtained.

Molecular weight of fucoidan-F was determined by means of a gelfiltration using Sephacryl S-500 and found to have a molecular weightdistribution around about 190,000.

Components of fucoidan-F were analyzed according to a method mentionedin Example 4.

Constituting sugars for fucoidan-F were fucose and galactose and theirmolar ratio was about 10:1. Uronic acid and other neutral sugars werenot substantially contained. Molar ratio of fucose to sulfate was about1:2.

A 1% fucoidan-F solution (16 ml) was mixed with 8 ml of 32 mU/ml of theabove-mentioned endo-fucoidan-lyase solution, 12 ml of 50 mM phosphatebuffer (pH 8.0), and 4 ml of 4 M sodium chloride and incubated at 25° C.for 48 hours. No degraded product by the reaction was noted and changeof fucoidan-F to low molecule was not noted as well.

Then, specific rotation of this freeze-dried fucoidan-F was measured bya high-speed highly-sensitive polarimeter SEPA-300 (mfd. by Horiba,Ltd.) and found to be −135°.

(2) The freeze-dried fucoidan VI mentioned in Example 1-(3) was used asfucoidan and the degraded product of fucoidan by microorganism wasprepared as follows.

Sixty grams of the above freeze-dried fucoidan VI were weighed,dissolved in 20 liters of artificial seawater (mfd. by JamarinLaboratory), 100 g of peptone and 2 g of yeast extract were added, themixture was charged in a 30-liter jar fermenter and sterilized,Flavobacterium sp. SA-0082 strain (FERM BP-5402) was inoculated andincubation was conducted for 26 hours under the conditions of incubatingtemperature of 24° C., pH 7, stirring rate of 125 rpm and aeration of 10liters/minute. The culture medium was centrifuged to remove the cells,treated with an ultrafiltration device equipped with a hollow fiberexcluding molecular weight of 30,000 or less to completely remove thelow molecular substances and centrifuged and filtrated to removeinsoluble matters and the degraded products of fucoidan by themicroorganism which were not excluded by a hollow fiber having anexcluding molecular weight of 30,000 or less were freeze-dried. Weightof the freeze-dried fucoidan degraded by the microorganism was 32 g.

(3) Freeze-dried fucoidan VI mentioned in Example 1-(3) was subjected toa method mentioned in Example 5-(1) to prepare fucoidan-U and thendegraded products of fucoidan-U using endo-fucoidan-lyase was preparedas follows.

Thus, 7 g of above-mentioned freeze-dried fucoidan VI was weighed anddissolved in 0.2 M calcium chloride. Then, 4,000 ml of column ofDEAE-Sepharose FF was equilibrated with 0.2 M calcium chloride. Afterthat, the above fucoidan dissolved as such was loaded on a column ofDEAE-Sepharose FF, well washed with 0.2 M calcium chloride and elutedwith a gradient of 0 to 4 M of sodium chloride. Among the elutedfractions, those where the sodium chloride concentrations were 0.05-0.8M were collected, desalted by means of dialysis and freeze-dried to givefucoidan-U.

Fucoidan-U (5 g) prepared as such was mixed with 250 ml of a 100 mMTris-hydrochloride buffer (pH 8.0), 250 ml of 0.8 M sodium chloride and0.15 ml of an endo-fucoidan-lyase solution (19 U/ml) produced byFlavobacterium sp. SA-0082 strain (FERM BP-5402) and made to react at25° C. for 80 hours.

The reaction mixture was subjected to ultrafiltration using anultrafiltering apparatus equipped with hollow fiber excluding molecularweight of 3,000 or less and, after the low-molecular substances werecompletely removed, the insoluble substance were eliminated by means ofcentrifugation and filtration to collect an enzymatically-degradedfucoidan-U which was not excluded by the hollow fiber having anexclusive molecular weight of 3,000 or less. Said fraction was desaltedby a Microacilyzer G3 and freeze-dried to give 2 g of freeze-driedenzymatically-degraded fucoidan-U.

(4) Apoptosis-inducing activity of fucoidan-U and fucoidan-F.

Human promyelocytic leukemia cells HL-60 (ATCC CCL-240) were incubatedat 37° C. in an RPMI 1640 medium (mfd. by Gibco) containing 10% of fetalcalf serum (mfd. by JPH Bioscience) treated at 56° C. for 30 minutes andthen suspended in an ASF 104 medium (mfd. by Ajinomoto Co., Ltd.) insuch a manner as to give a concentration of 5×10⁴ cells/900 M 1. Thenthe suspension was pipetted into a six-well plate (mfd. by Falcon) at aratio of 4.5 ml/well. Each of fucoidan-F and fucoidan-U prepared inExample 5-(1) was dissolved in a 30 mM HEPES buffer (pH 7) containing120 mM of sodium chloride so as to make the concentration 10 mg/mlfollowed by subjecting to a treatment in an autoclave at 121° C. for 20minutes and 100 μl of the resulting one was added to each of thealready-prepared suspensions followed incubating at 37° C. in thepresence of 5% of carbon dioxide. As a control, only the above bufferwas added in the same amount followed by subjecting to the sameincubation. Viable cell numbers after 16 hour and 40 hours frominitiation of the incubation were counted according to a methodmentioned in “Techniques in Tissue Culture” (second edition) (publishedby Asakura Shuppan, edited by Japan Tissue Culture Society, pages26-28). Thus, the counting was conducted by a method where dyeing wascarried out with Trypan Blue on a hemocytometer.

The results are given in FIG. 5. Thus, FIG. 5 shows the relation betweenthe incubation time and viable cell numbers in the culture medium whenfucoidan-U or fucoidan-F was added to a culture medium of HL-60 cells tomake its concentration 1 mg/ml. An abscissa shows incubation time(hours) while an ordinate shows viable cell numbers in the culturemedium. With regard to the type of the sulfated fucose-containingpolysaccharides, open circles stand for fucoidan-U while black circlesstand for fucoidan-F. Incidentally, viable cell numbers in the culturemedium of the control (no sample added) after 16 hours and 40 hours frominitiation of the incubation were 7×10⁴ cells/ml and 1.4×10⁵ cells/ml,respectively.

As a result, it was found that apoptosis was induced in HL-60 cells byfucoidan-F and fucoidan-U whereupon the rate of proliferation of cellswas suppressed.

Each of the fucoidan which was degraded by the microorganism prepared inExample 5-(2) and fucoidan-U which was degraded by enzyme prepared inExample 5-(3) hereinabove was dissolved in a 30 mM HEPES buffer (pH 7)containing 120 mM of sodium chloride to make its concentration 10 mg/mlfollowed by subjecting to a treatment in an autoclave at 121° C. for 20minutes and the apoptosis-inducing activity achieved thereby wasmeasured according to the above-mentioned method whereupon the sameresults as in the case of fucoidan-U shown in FIG. 5 were obtained.

EXAMPLE 6

Green tea was prepared by a conventional method using 10 g of green tealeaves, 0.2 g of vitamin C and 1,000 ml of deionized water. In theproduct 1 of the present invention, fucoidan II of Example 1 was usedand 30 mg of fucoidan per 100 ml of the product was added. In theproduct 2 of the present invention, a mixture (containing 60% offucoidan) of fucoidan II of Example 1 and an extract of seaweed with hotwater by a conventional method [Kjellmaniella crassifolia was extractedwith hot water at 95° C. for one hour by a conventional method and theextract was treated with active carbon and freeze-dried (containing 50%of fucoidan); the same material was used in the following Examples aswell] was used and added in such an amount that corresponded to 30 mg offucoidan per 100 ml of the final product. A control was that wherenothing was added. An organoleptic test was conducted by 20 panelists bymeans of a five-step evaluation (where 5 being good while 1 being bad)in terms of feel on tongue, balance of taste, refreshingness of tasteand feel on throat and the averages of the results are shown in Table 4.

TABLE 4 Organoleptic Evaluations Product 1 Product 2 Control Feel ontongue Mellowness 4.5 3.2 2.1 Smoothness 4.7 2.9 2.0 Balance of taste4.2 2.7 2.0 Refreshingness of taste 4.0 2.5 2.0 Feel on throat 4.5 3.12.3 Total evaluation 4.4 2.9 2.1

From Table 4, the evaluations were that, as compared with the control,the products 1 and 2 of the present invention showed better feel ontongue, better balance and refreshingness of taste, prominent aroma andtaste of the tea and excellent balance of taste.

EXAMPLE 7

Nutritive drink was prepared by a conventional method according to aformulation as shown in Table 5.

TABLE 5 Formulation Fructose-Glucose-Liquid Sugar 150 g Purified honey 2g Guarana extract 1 g Korean ginseng extract 0.1 g Royal jelly 0.05 gVitamin C 0.5 g Nicotinamide 0.1 g Vitamin B₁ hydrochloride 0.02 gVitamin B₆ hydrochloride 0.02 g L-Phenylalanine 0.04 g L-Isoleucine 0.01g Citric acid 1.5 g Perfume 2 g Deionized water balance Total 1,000 ml

In a product 3 of the present invention, fucoidan-U of Example 4 wasused and 40 mg of fucoidan per 100 ml of the final product was added. Ina product 4 of the present invention, a mixture (containing 67% offucoidan) of fucoidan-U of Example 4 and an extract of seaweed with hotwater was used and the amount corresponding to 40 mg of fucoidan per 100ml of the final product was added. The control was that where nofucoidan was added. An organoleptic test was conducted by the samemanner as in Example 6. The results are shown in Table 6.

TABLE 6 Organoleptic Evaluations Product 3 Product 4 Control Feel ontongue Mellowness 3.0 2.5 1.8 Smoothness 3.5 2.4 1.5 Balance of taste3.6 3.2 3.0 Refreshingness of taste 3.8 3.0 2.8 Feel on throat 3.7 3.43.0 Total evaluation 3.4 2.9 2.4

From Table 6, it is noted that the products 3 and 4 of the presentinvention were quite refreshing beverages having improved feel ontongue, balance of taste, refreshingness of taste and feel on throat ascompared with the control.

EXAMPLE 8

Fucoidan drink of a concentrated type was prepared by a conventionalmethod according to a formulation given in Table 7.

TABLE 7 Formulation Maltose liquid sugar (kg) 30.00 Ume (Japaneseapricot) powder (kg) 0.50 ⅕ Transparent lemon juice (kg) 2.00 Pectin(kg) 3.00 Citric acid anhydride (kg) 0.19 Vitamin C (kg) 0.20 Perfume(kg) 1.20 Deionized water balance Total (liter) 1000 (pH of the product:3.1)

The product 5 of the present invention used fucoidan V mentioned inExample 1-(2) and 400 mg of fucoidan per 100 ml of the final product wasadded. The product 6 of the present invention used a mixture (containing80% of fucoidan) of fucoidan V and an extract of seaweed with hot waterand an amount corresponding to 400 mg of fucoidan per 100 ml of thefinal product was added. Since the products were drinks of aconcentrated type, control 1 where 400 mg of duran gum (a thickener)/100ml was added and control 2 where 400 mg of xanthan gum/100 ml was addedwere prepared as controls. They were subjected to an organoleptic testby the same manner as in Example 6 and the results are given in Table 8.

TABLE 8 Organoleptic Evaluations Product Product Control Control 5 6 1 2Feel on tongue Mellowness 4.0 3.7 3.5 3.4 Smoothness 4.1 3.6 3.3 3.2Balance of taste 4.0 3.8 3.2 3.1 Refreshingness of taste 3.5 3.3 3.1 3.3Feel on throat 3.7 3.6 3.2 3.3 Total evaluation 4.0 3.7 3.3 3.2

From Table 8, it is noted that the products 5 and 6 of the presentinvention showed better feel on tongue and balance of taste as comparedwith the controls 1 and 2 giving good products having excellent taste asdrinks of a concentrated type. Further, fucoidan VI mentioned in Example1-(3) was used and 400 mg of fucoidan per 100 ml of the product wasadded to prepare a drink of a concentrated type, which gave the sameresult. Furthermore, each of the degraded products prepared in Examples5-(2) and (3) was used and 400 mg of said degraded product per 100 ml ofthe final product was added to prepare the drinks of the concentratedtype, which gave the same result.

EXAMPLE 9

An alcoholic beverage was prepared by a conventional method inaccordance with the formulation as shown in Table 9.

TABLE 9 Formulation Concentrated juice of frozen Citrus unshu (45 Brixdegree) 110 g Granulated sugar 80 g Citric acid 2 g Sodium citrate 0.5 gOrange essence 2 g 5% (v/v) aqueous solution of alcohol balance Total1,000 ml Note: The beverage prepared as such was cooled at 5° C. andthen carbonic acid was made contained therein by means of a soda siphon.

The product 7 of the present invention used fucoidan II of Example 1 and35 mg of fucoidan per 100 ml of the final product was added. The product8 of the present invention used a mixture (containing 55% of fucoidan)of fucoidan II of Example 1 and an extract of seaweed with hot water andthe amount corresponding to 35 mg of fucoidan was added. As a control,to which no fucoidan was added was used. An organoleptic test wasconducted by the same manner as in Example 6 and the results are givenin Table 10.

TABLE 10 Organoleptic Evaluations Product 7 Product 8 Control Feel ontongue Mellowness 4.2 3.3 2.7 Smoothness 4.3 3.1 2.8 Balance of taste4.0 3.5 2.5 Refreshingness of taste 3.9 3.0 2.7 Feel on throat 3.5 2.72.1 Total evaluation 4.0 3.1 2.6

As shown in Table 10, the products 7 and 8 of the present invention hadimproved feel on tongue, balance of taste, refreshingness of taste andfeel on throat as compared with the control. Particularly in the product7, acid taste became mild giving a taste of well-ripened mandarinorange.

EXAMPLE 10

A sports drink was prepared by a conventional method according to aformulation of Table 11.

TABLE 11 Formulation Glucose 48 g Fructose 7.8 g Citric acid 1.4 gSodium citrate 1.0 g Pure salt 0.3 g Calcium lactate 0.1 g Magnesiumchloride 0.1 g Vitamin C 0.2 g Vitamin B1 hydrochloride 0.02 g Lemonlime perfume 2 g Deionized water balance Total 1,000 g

In the product 9 of the present invention, fucoidan-U of Example 4 wasused and 30 mg of fucoidan per 100 g of the product was added. In theproduct 10 of the present invention, a mixture (containing 75% offucoidan) of fucoidan-U of Example 4 and an extract of seaweed with hotwater was used and an amount corresponding to 30 mg of fucoidan wasadded. As a control, to which no fucoidan was added was used. They weresubjected to an organoleptic test by the same manner as in Example 6.The results are given in Table 12.

TABLE 12 Organoleptic Evaluations Product 9 Product 10 Control Feel ontongue Mellowness 4.5 3.8 2.5 Smoothness 4.3 3.5 2.3 Balance of taste3.9 3.5 3.2 Refreshingness of taste 3.7 3.2 2.9 Feel on throat 4.0 3.52.8 Total evaluation 4.0 3.5 2.7

From Table 12, it was noted that, as compared with the control, theproducts 9 and 10 of the present invention had better feel on tongue,feel on throat and balance of taste, showed excellent balance among thecomponents and gave significant effect of mature taste.

EXAMPLE 11

In order to prepare a plum brandy with fruit, 1,440 g of 75 (w/w) %fructose-glucose-liguid sugar, 670 ml of 95 (v/v) % alcohol and 340 mlof water were mixed in a five-liter bottle equipped with a cap and 1 kgof unripe plum was placed therein.

In the product 11 of the present invention, fucoidan III of Example 2was used in charging the materials and 40 mg of fucoidan per 100 ml ofthe final product was used. In the product 12 of the present invention,a mixture (containing 70% of fucoidan) of fucoidan III and an extract ofseaweed with hot water and an amount corresponding to 40 mg of fucoidanper 100 ml of the final product as added. A control is that to which nofucoidan was added.

Each bottle was capped and allowed to stand at room temperature for twomonths with occasional stirring. After that, 1,020 ml of a 28 (v/v) %aqueous alcohol was added thereto and mixed therewith and maturing wascontinued for two months more to prepare a plum brandy.

Each of the resulting matured plum brandy products was subjected to anorganoleptic test by the same manner as in Example 6. The results aregiven in Table 13.

TABLE 13 Organoleptic Evaluations Product 11 Product 12 Control Feel ontongue Mellowness 4.5 3.2 2.5 Smoothness 4.2 3.3 2.6 Balance of taste4.6 4.0 2.4 Refreshingness of taste 3.8 3.5 2.7 Feel on throat 4.2 3.42.8 Total evaluation 4.3 3.5 2.6

From Table 13, the products 11 and 12 of the present invention hadbetter feel on tongue and feel on throat as compared with the controland showed mature and well-balanced taste like in the product which wasmatured for long term.

EXAMPLE 12

In the product 13 of the present invention, homogenized normal milk(containing 88.6 w/v % water, 2.8 w/v % of protein, 3.5 v/w % of fat,4.5 w/v % of lactose and 0.8 w/v % of ash) and fucoidan I of Example 1were used and 30 mg of fucoidan per 100 ml of the final product wasadded. In the product 14 of the present invention, a mixture (containing80% of fucoidan) of fucoidan I of Example 1 and an extract of seaweedwith hot water was used and an amount corresponding to 30 mg of fucoidanwas added. The control was that where no fucoidan was added. Anorganoleptic test was conducted by the same manner as in Example 6 andthe results are shown in Table 14.

TABLE 14 Organoleptic Evaluations Product 13 Product 14 Control Feel ontongue Mellowness 3.6 3.0 2.1 Smoothness 3.5 2.9 2.5 Balance of taste4.0 3.6 3.0 Refreshingness of taste 4.2 2.8 2.4 Feel on throat 3.9 3.32.1 Total evaluation 3.8 3.1 2.4

From Table 14, it is noted that, as compared with the control, theproducts 13 and 14 of the present invention had improved feel on tongueand balance of taste, showed better refreshing of the taste (as comparedwith the feel in the control as if milk hangs around the tongue; i.e.poor refreshing of the taste) whereupon the resulting milk products canbe taken favorably.

EXAMPLE 13

Bean milk was prepared from soybean by a conventional method andcoagulated with a coagulator to prepare a common soybean curd.

In the product 15 of the present invention, fucoidan IV of Example 2 wasused in the bean milk and an amount of 40 mg of fucoidan per 100 g ofthe final product was added. In the product 16 of the present inventiona mixture (containing 60% of fucoidan) of fucoidan IV of Example 2 andan extract of seaweed with hot water was used and an amountcorresponding to 40 mg of fucoidan per 100 ml of the final product wasadded. A control was that where no additive was used. An organoleptictest was conducted by the same manner as in Example 6. The results aregiven in Table 15.

TABLE 15 Organoleptic Evaluations Product 15 Product 16 Control Feel ontongue Mellowness 4.0 3.2 2.8 Smoothness 3.8 3.1 2.5 Texture 3.6 3.2 2.8Total evaluation 3.8 3.2 2.7

From Table 15, it is noted that, as compared with the control, theproducts 15 and 16 of the present invention showed improved feel ontongue and also had a delicate feel on tongue like the soybean cakeprepared by compressing with a silk cloth whereby the total feel oneating was significantly improved.

EXAMPLE 14

As to confectionery products, chocolate cream, candy and orange jellywere prepared as follows.

Chocolate cream was prepared by kneading two egg yolks, 125 ml of cow'smilk, 10 g of wheat flour and 30 g of sugar with warming.

Candy was prepared as follows. Thus, 1.2 kg of sugar and 0.8 kg of syrupwere mixed and dissolved in a dissolver (110° C.), boiled up to 120-130°C. by a cooker to make the water content 2% or less and then 16.3 g oflactic acid (a 50% by weight solution), 10.1 g of malic acid, 5.0 g ofcalcium carbonate and an appropriate amount of perfume were added toprepare the candy.

Orange jelly was prepared as follows. Thus, 9 g of carrageenan was mixedwith 180 g of granulated sugar and 800 ml of water was added theretofollowed by mixing and dissolving with heating. To this were added 10 gof concentrated fruit juice of unshu-mikan (Citrus unshu), 2 g of citricacid, 1.5 g of sodium citrate, 2 g of orange aroma and 1 g of perfume togive a final product.

In each of the products 17 (chocolate cream), 18 (candy) and 19 (orangejelly) of the present invention, fucoidan-U of Example 4 was used and 20mg of fucoidan per 100 g of the final product was added. In each of theproducts 20 (chocolate cream), 21 (candy) and 22 (orange jelly) of thepresent invention, a mixture (containing 60% of fucoidan) of fucoidan-Uof Example 4 and an extract of seaweed with hot water was used and anamount corresponding to 20 mg of fucoidan per 100 g of the final productwas added. A control was that where no additive was used. Anorganoleptic test was conducted and the results are given in Table 16.

TABLE 16 Organoleptic Evaluations Tongue Feel Texture Total EvalnProducts Con- Products Con- Products Con- 17 20 trol 17 20 trol 17 20trol Choco- 3.5 2.9 2.4 3.7 3.2 2.6 3.6 3.0 2.5 late Cream Products Con-Products Con- Products Con- 18 21 trol 18 21 trol 18 21 trol Candy 3.83.1 2.3 3.6 3.2 2.4 3.7 3.1 2.3 Products Con- Products Con- ProductsCon- 19 22 trol 19 22 trol 19 22 trol Orange 4.2 3.0 2.2 4.1 2.9 2.3 4.12.9 2.2 Jelly

From Table 16, it is noted that the products 17/18/19 and 20/21/22showed improved smoothness in terms of feel on tongue giving a mild feelon eating and exhibited better total evaluations too as compared withcontrols for each of them.

EXAMPLE 15

As the meat paste product, kamaboko using fish paste and sausage usinganimal meat were prepared.

In the case of kamaboko, 100 g of water and 20 g of salt were added to 1kg of ground meat (grade: SA) of walleye pollak, the mixture wasdisintegrated/kneaded for 15 minutes, 40 g each of it was packed in avinyl bag, stored overnight at 5° C. and steamed at ordinary pressure toprepare steamed kamaboko (boiled fish paste).

In the case of sausage, 2 kg of pork and 700 g of lard were ground usinga plate having pores of 5 mm diameter, then mixed with 7 g of pepper, 3g of sage and 1 g of mace and the mixture was subjected to cutting andcasing using a pig intestine of 2 cm diameter. This is steamed for 15minutes to prepare sausage.

Fucoidan I of Example 1 was used and 50 mg of fucoidan per 100 g of theproduct was added to kamaboko (the product 23) prior todisintegrating/kneading or to sausage (the product 24) prior to cutting.In the case of the kamaboko of the product 25 and the sausage of theproduct 26, a mixture (containing 67% of fucoidan) of fucoidan I of theExample 1 and an extract of seaweed with hot water was used and anamount corresponding to 50 mg of fucoidan per 100 g of the final productwas added. A control was that where no additive was used. Anorganoleptic test was conducted by the same manner as in Example 6 andthe results are given in Table 17.

TABLE 17 Organoleptic Evaluations Tongue Feel Texture Total EvalnProducts Con- Products Con- Products Con- 23 25 trol 23 25 trol 23 25trol Kama- 3.8 3.4 2.7 3.7 3.0 2.6 3.7 3.2 2.6 boko Products Con-Products Con- Products Con- 24 26 trol 24 26 trol 24 26 trol Sausage 3.83.1 2.8 3.6 3.1 2.5 3.7 3.1 2.6

It is noted from Table 17 that, as compared with the control, theproducts 23/25 (kamaboko) and 24/26 (sausage) of the present inventionshowed milder feel on tongue and improved elasticity in texture.

EXAMPLE 16

Ramen (a kind of Chinese noodles) was prepared. Thus, 25.4 g of sodiumlactate (50 w/w % solution), 9.4 g of sodium malate and 10 g of calciumcarbonate were added to 4 kg of a special flour mixture for ramen (awheat flour containing a specific alkaline salt), then 1.6 liters ofwater was further added thereto and the mixture was well mixed up. Thiswas made into noodles using a domestic noodle manufacturing machine(mfd. by Sanyo Electric).

In the product 27 of the present invention, fucoidan II of Example 1 wasused and 40 mg of fucoidan per 100 g of the product was added. In theproduct 28 of the present invention, a mixture (containing 60% offucoidan) of fucoidan II of Example 1 and an extract of seaweed with hotwater was used and an amount corresponding to 40 mg fucoidan per 100 gof the final product was added prior to addition of water. The controlwas that which contained no additive.

The resulting ramen noodles were cooked by a conventional method and anorganoleptic test was conducted for the cooked one by the same method asin Example 6. The results are given in Table 18.

TABLE 18 Organoleptic Evaluations Product 27 Product 28 Control Feel ontongue 3.8 3.2 2.5 Texture 3.5 3.0 2.8 Total evaluation 3.6 3.1 2.6

From Table 18, it is noted that, as compared with the control, theproducts 27 and 28 of the present invention showed improved feel ontongue and had elastic texture giving a good biting nature by toothwhereby the total evaluation was in a high degree.

EXAMPLE 17

Bread and Chinese manju (dim sum or bun with a filling) were prepared bya conventional method.

Compounding and preparing conditions for the bread and the coating forChinese manju are given in Tables 19 and 20, respectively.

TABLE 19 Compounding and Preparing Conditions for Bread (Formulation forSponge Dough Preparation) Wheat flour 70 parts by weight Yeast  2 Yeastfood  0.1 Water 40 (Formulation for Final Kneading Operation) Wheatflour 30 parts by weight Sugar  5 Salt  2 Shortening  5 Casein  0.5Water 25 (Preparing Conditions) Fermenting time:  4 hours (at 27° C.temp and 75% humidity) Proofing time: 40 minutes (at 38° C. temp and 85%humidity) Baking time: 35 minutes (at 210° C.)

TABLE 20 Compounding and Preparing Conditions for Coating of ChineseManju (Compounding Formulation for the Coating) Wheat flour 100 parts byweight Sugar  15 Salt  0.8 Baking powder  1 Lard  5 Yeast  3.5 Water 43.5 (Preparing Condition) Proofing time: 90 minutes (at 45° C. tempand 75% humidity) Steaming time: 15 minutes

In the products 29 (bread) and 30 (coating for Chinese manju) of thepresent invention, fucoidan IV of Example 2 was used and 35 mg offucoidan per 100 g of the bread or of the coating was added. In theproducts 31 (bread) and 32 (coating for Chinese manju), a mixture(containing 80% of fucoidan) of fucoidan IV of Example 2 and an extractof seaweed with hot water was used and an amount corresponding to 35 mgof fucoidan was added during the compounding stage of the materials.Controls are those where no additive was used.

The resulting bread and coating of Chinese manju were wrapped with aclear-plastic wrap and allowed to stand at 5° C. for 24 hours. Anorganoleptic test was conducted using the bread and the coating forChinese manju after allowing to stand as such by the same manner as inExample 6 and the results are as shown in Table 21.

TABLE 21 Organoleptic Evaluations Tongue Feel Texture Total EvalnProducts Con- Products Con- Products Con- 29 31 trol 29 31 trol 29 31trol Bread 3.3 2.0 1.5 3.0 1.8 1.3 3.1 1.9 1.4 Products Con- ProductsCon- Products Con- 30 32 trol 30 32 trol 30 32 trol Coating 2.8 2.1 1.63.4 1.7 1.1 3.1 1.9 1.3

When bread and coating for Chinese manju are allowed to stand at lowtemperature, their moist feels are deteriorated giving a dry feel oneating. As shown in Table 21 however, the products 29/31 and 30/32 ofthe present invention showed, as compared with the controls for each ofthem, better feel on tongue and texture after allowing to standresulting in good inhibition for deterioration in feel on eating wherebya high effect of maintaining their good feel on eating was confirmed.

EXAMPLE 18

In the product 33 of the present invention, a conventionally-preparedsake (Japanese rice wine) and fucoidan-U of Example 4 were used and 25mg of fucoidan per 100 ml of the final product was added. In the product34 of the present invention, a mixture (containing 70% of fucoidan) offucoidan-U of Example 4 and an extract of seaweed with hot water wasused and an amount corresponding to 25 mg of fucoidan per 100 ml of thefinal product was added. A control was that where no additive was used.

An organoleptic test was conducted by the same manner as in Example 6.Taste and aroma were added to the items for the evaluations and theresults are given in Table 22.

TABLE 22 Organoleptic Evaluations Product 33 Product 34 Control Taste3.1 3.0 3.0 Aroma 2.9 2.8 2.9 Feel on tongue Mellowness 3.9 3.2 2.5Smoothness 4.2 3.5 2.8 Balance of taste 3.5 3.1 2.9 Refreshingness oftaste 3.6 3.2 2.7 Feel on throat 3.8 3.0 2.6 Total evaluation 3.7 3.12.8

From Table 22, it is noted that, as compared with the control, theproducts 33 and 34 of the present invention showed better feel ontongue, particularly in terms of smoothness, and exhibited improvedrefreshingness of taste and feel on throat as well whereby an effect ofimproving the feel on eating as a luxury has been achieved.

EXAMPLE 19

Conventionally-prepared mirin (a sweet sake) and fermented seasoningwere used in this example. Thus, in the products 35 (mirin) and 37(fermented seasoning) of the present invention, fucoidan III of Example2 was used and 30 mg of fucoidan per 100 ml of the final product wasadded. In the products 36 (mirin) and 38 (fermented seasoning), amixture (containing 67% of fucoidan of fucoidan III of Example 2 and anextract of seaweed with hot water was used and an amount correspondingto 30 mg of fucoidan per 100 ml of the final product was added. Controlsare those where no additive was used.

An organoleptic test was conducted by the same manner as in Example 6.Taste and aroma were added to items for the evaluations and the resultsare given in Table 23.

TABLE 23 Organoleptic Evaluations Mirin Fermented Seasoning ProductsCon- Products Con- 35 37 trol 36 38 trol Taste 2.9 2.8 2.8 2.7 2.6 2.7Aroma 2.7 2.4 2.7 2.4 2.1 2.4 Feel on tongue Mellowness 3.9 3.0 2.5 3.72.8 2.6 Smoothness 4.1 3.2 2.7 3.8 2.9 2.4 Balance of taste 3.4 3.2 3.12.9 2.5 2.4 Refreshingness of taste 3.5 3.1 3.0 3.2 2.8 2.3 Totalevaluation 3.4 3.0 2.8 3.1 2.6 2.5

It is noted from Table 23 that, as compared with each of the controls,the mirin products (35/37) and the fermented seasoning product (36/38)of the present invention showed improved feel on tongue (particularly interms of mellowness and smoothness) and balance of taste whereby theproducts were effective as seasonings in improving the feel on eating ofthe materials in cooking.

EXAMPLE 20

Fish powder (4.7 kg), 0.8 kg of dried laver, 2.5 kg of sesame, 1.0 kg ofsalt and 0.5 kg of sodium glutamate were mixed followed by granulatingby a conventional method to prepare furikake (seasoned fish flour).

In the product 39 of the present invention, fucoidan-U of Example 4 wasused and 1, 000 mg of fucoidan per 100 g of the final product was added.In the product 40 of the present invention, a mixture (containing 60% offucoidan) of fucoidan-U of Example 4 and an extract of seaweed with hotwater was used and an amount corresponding to 1,000 mg of fucoidan per100 g of the final product was added. The control was that where noadditive was used. The product was sprinkled over boiled rice and anorganoleptic test on the feel upon eating was conducted by the samemanner as in Example 6.

The result was that, as compared with the control, the products 39 and40 of the present invention well matched with the boiled rice in mouthgiving good feel on tongue and showing no coarse feel whereby, as awhole, they were found to improve the quality of the seasoned fishflour.

MERIT OF THE INVENTION

The food or beverage of the present invention contains a lot of fucoidanhaving a physiological activity and/or degraded product thereof and, dueto an apoptosis-inducing action, etc. of said fucoidan, the product is ahealthy or functional food or beverage which shows an effect ofpreventing the carcinogenesis and suppressing the cancer upon taking itand, particularly, the product is a food or beverage containingfunctional marine fiber which is useful for maintaining good health ofstomach and intestine.

Fucoidan of the present invention, particularly fucoidan from seaweed,is a very good material because it is available at low cost and in largequantities from edible seaweed plants, i.e. edible substance and has ahigh safety. Moreover, in accordance with the present invention, it ispossible to offer fucoidan where the alginic acid content is reduced oreliminated. Said fucoidan is capable of improving feel on eating such asfeel on tongue, refreshingness of taste, matching of taste, texture,etc. without deteriorating the inherent good nature of food and beverageand is also capable of maintaining the good nature in view of feel uponeating whereupon it is extremely useful in the manufacture of food andbeverage.

What is claimed is:
 1. A method of improving feel of a food or beverageduring eating, said method comprising adding fucoidan derived fromKjellmaniella crassifolia to the food or beverage.
 2. The methodaccording to claim 1, wherein the food or beverage comprises flicoidanderived from Kjellmaniella crassifolia and substantially no algins. 3.The method according to claim 1, wherein the fucoidan is afucoidan-containing extract.
 4. The method according to claim 3, whereinthe fucoidan-containing extract is extracted from Kjellmaniellacrassifolia with a calcium salt.
 5. The method according to claim 3,wherein the fucoidan-containing extract is extracted from Kjellmaniellacrassifolia with an alkaline solution and a calcium salt.
 6. The methodaccording to claim 3, wherein the fucoidan-containing extract isextracted from Kjellmaniella crassifolia with an alkaline solution andacidification.
 7. The method according to claim 1, wherein the fucoidanis prepared from Kjellmaniella crassifolia.
 8. The method according toclaim 1, wherein the fucoidan contains uronic acid and has a molecularweight which can be lowered by an endo-fucoidan-lyase produced byFlavobacterium sp. SA-0082 (FERM BP-5402) to produce one or morecompounds having the following formulae (I), (II) or (III):


9. The method according to claim 1, wherein the fucoidan containssubstantially no uronic acid, and has a molecular weight which cannot besubstantially lowered by an endo-flicoidan-lyase produced byFlavobacterium sp. SA-0082 (FERM BP-5402).
 10. The method according toclaim 1, wherein the fucoidan is degraded.
 11. The method according toclaim 1, wherein the amount of flicoidan calculated as flicose is 0.001w/w % or more.
 12. The method according to claim 1, wherein the amountof fucoidan is 10-200 mg w/v %.
 13. The method according to claim 1,wherein the food or beverage further comprises algins and wherein theweight percentage of fucoidan to that of algins and fucoidan is 27% ormore.